PhanerochaeteV1, Main, Exploration, bibRecord, 000575

Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.

Identifieur interne : 000575 ( Main/Exploration ); précédent : 000574; suivant : 000576

Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.

Auteurs : Nomathemba Loice Chigu [Japon] ; Sinji Hirosue ; Chie Nakamura ; Hiroshi Teramoto ; Hirofumi Ichinose ; Hiroyuki Wariishi

Source :

Applied microbiology and biotechnology [ 1432-0614 ] ; 2010.

RBID : pubmed:20508934

Descripteurs français

English descriptors

KwdEn :
- Anthracenes (metabolism), Anthraquinones (metabolism), Cytochrome P-450 Enzyme System (metabolism), Gene Expression Profiling (MeSH), Metabolic Networks and Pathways (MeSH), Mixed Function Oxygenases (metabolism), Oligonucleotide Array Sequence Analysis (MeSH), Phanerochaete (enzymology), Phanerochaete (metabolism).
MESH :
- chemical , metabolism : Anthracenes, Anthraquinones, Cytochrome P-450 Enzyme System, Mixed Function Oxygenases.
- enzymology : Phanerochaete.
- metabolism : Phanerochaete.
- Gene Expression Profiling, Metabolic Networks and Pathways, Oligonucleotide Array Sequence Analysis.

Abstract

Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.

DOI: 10.1007/s00253-010-2616-1
PubMed: 20508934

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000553
to stream Main, to step Curation: 000553

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Chigu, Nomathemba Loice" sort="Chigu, Nomathemba Loice" uniqKey="Chigu N" first="Nomathemba Loice" last="Chigu">Nomathemba Loice Chigu</name>
<affiliation wicri:level="4"><nlm:affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581</wicri:regionArea>
<orgName type="university">Université de Kyūshū</orgName>
<placeName><settlement type="city">Fukuoka</settlement>
<region type="province">Kyūshū</region>
<region type="prefecture">Préfecture de Fukuoka</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hirosue, Sinji" sort="Hirosue, Sinji" uniqKey="Hirosue S" first="Sinji" last="Hirosue">Sinji Hirosue</name>
</author>
<author><name sortKey="Nakamura, Chie" sort="Nakamura, Chie" uniqKey="Nakamura C" first="Chie" last="Nakamura">Chie Nakamura</name>
</author>
<author><name sortKey="Teramoto, Hiroshi" sort="Teramoto, Hiroshi" uniqKey="Teramoto H" first="Hiroshi" last="Teramoto">Hiroshi Teramoto</name>
</author>
<author><name sortKey="Ichinose, Hirofumi" sort="Ichinose, Hirofumi" uniqKey="Ichinose H" first="Hirofumi" last="Ichinose">Hirofumi Ichinose</name>
</author>
<author><name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20508934</idno>
<idno type="pmid">20508934</idno>
<idno type="doi">10.1007/s00253-010-2616-1</idno>
<idno type="wicri:Area/Main/Corpus">000553</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000553</idno>
<idno type="wicri:Area/Main/Curation">000553</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000553</idno>
<idno type="wicri:Area/Main/Exploration">000553</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Chigu, Nomathemba Loice" sort="Chigu, Nomathemba Loice" uniqKey="Chigu N" first="Nomathemba Loice" last="Chigu">Nomathemba Loice Chigu</name>
<affiliation wicri:level="4"><nlm:affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581</wicri:regionArea>
<orgName type="university">Université de Kyūshū</orgName>
<placeName><settlement type="city">Fukuoka</settlement>
<region type="province">Kyūshū</region>
<region type="prefecture">Préfecture de Fukuoka</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hirosue, Sinji" sort="Hirosue, Sinji" uniqKey="Hirosue S" first="Sinji" last="Hirosue">Sinji Hirosue</name>
</author>
<author><name sortKey="Nakamura, Chie" sort="Nakamura, Chie" uniqKey="Nakamura C" first="Chie" last="Nakamura">Chie Nakamura</name>
</author>
<author><name sortKey="Teramoto, Hiroshi" sort="Teramoto, Hiroshi" uniqKey="Teramoto H" first="Hiroshi" last="Teramoto">Hiroshi Teramoto</name>
</author>
<author><name sortKey="Ichinose, Hirofumi" sort="Ichinose, Hirofumi" uniqKey="Ichinose H" first="Hirofumi" last="Ichinose">Hirofumi Ichinose</name>
</author>
<author><name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name>
</author>
</analytic>
<series><title level="j">Applied microbiology and biotechnology</title>
<idno type="eISSN">1432-0614</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthracenes (metabolism)</term>
<term>Anthraquinones (metabolism)</term>
<term>Cytochrome P-450 Enzyme System (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Metabolic Networks and Pathways (MeSH)</term>
<term>Mixed Function Oxygenases (metabolism)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Phanerochaete (enzymology)</term>
<term>Phanerochaete (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Anthracènes (métabolisme)</term>
<term>Anthraquinones (métabolisme)</term>
<term>Cytochrome P-450 enzyme system (métabolisme)</term>
<term>Mixed function oxygenases (métabolisme)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Séquençage par oligonucléotides en batterie (MeSH)</term>
<term>Voies et réseaux métaboliques (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anthracenes</term>
<term>Anthraquinones</term>
<term>Cytochrome P-450 Enzyme System</term>
<term>Mixed Function Oxygenases</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anthracènes</term>
<term>Anthraquinones</term>
<term>Cytochrome P-450 enzyme system</term>
<term>Mixed function oxygenases</term>
<term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term>
<term>Metabolic Networks and Pathways</term>
<term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Voies et réseaux métaboliques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20508934</PMID>
<DateCompleted><Year>2010</Year>
<Month>10</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2012</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>87</Volume>
<Issue>5</Issue>
<PubDate><Year>2010</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Applied microbiology and biotechnology</Title>
<ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>1907-16</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-010-2616-1</ELocationID>
<Abstract><AbstractText>Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chigu</LastName>
<ForeName>Nomathemba Loice</ForeName>
<Initials>NL</Initials>
<AffiliationInfo><Affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hirosue</LastName>
<ForeName>Sinji</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Nakamura</LastName>
<ForeName>Chie</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Teramoto</LastName>
<ForeName>Hiroshi</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ichinose</LastName>
<ForeName>Hirofumi</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wariishi</LastName>
<ForeName>Hiroyuki</ForeName>
<Initials>H</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2010</Year>
<Month>05</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Appl Microbiol Biotechnol</MedlineTA>
<NlmUniqueID>8406612</NlmUniqueID>
<ISSNLinking>0175-7598</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000873">Anthracenes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000880">Anthraquinones</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9035-51-2</RegistryNumber>
<NameOfSubstance UI="D003577">Cytochrome P-450 Enzyme System</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.-</RegistryNumber>
<NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EH46A1TLD7</RegistryNumber>
<NameOfSubstance UI="C034020">anthracene</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>FP0FJ7K744</RegistryNumber>
<NameOfSubstance UI="C004522">anthrone</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000873" MajorTopicYN="N">Anthracenes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000880" MajorTopicYN="N">Anthraquinones</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003577" MajorTopicYN="N">Cytochrome P-450 Enzyme System</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year>
<Month>01</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2010</Year>
<Month>04</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2010</Year>
<Month>03</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2010</Year>
<Month>5</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2010</Year>
<Month>5</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2010</Year>
<Month>10</Month>
<Day>15</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">20508934</ArticleId>
<ArticleId IdType="doi">10.1007/s00253-010-2616-1</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Japon</li>
</country>
<region><li>Kyūshū</li>
<li>Préfecture de Fukuoka</li>
</region>
<settlement><li>Fukuoka</li>
</settlement>
<orgName><li>Université de Kyūshū</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Hirosue, Sinji" sort="Hirosue, Sinji" uniqKey="Hirosue S" first="Sinji" last="Hirosue">Sinji Hirosue</name>
<name sortKey="Ichinose, Hirofumi" sort="Ichinose, Hirofumi" uniqKey="Ichinose H" first="Hirofumi" last="Ichinose">Hirofumi Ichinose</name>
<name sortKey="Nakamura, Chie" sort="Nakamura, Chie" uniqKey="Nakamura C" first="Chie" last="Nakamura">Chie Nakamura</name>
<name sortKey="Teramoto, Hiroshi" sort="Teramoto, Hiroshi" uniqKey="Teramoto H" first="Hiroshi" last="Teramoto">Hiroshi Teramoto</name>
<name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name>
</noCountry>
<country name="Japon"><region name="Kyūshū"><name sortKey="Chigu, Nomathemba Loice" sort="Chigu, Nomathemba Loice" uniqKey="Chigu N" first="Nomathemba Loice" last="Chigu">Nomathemba Loice Chigu</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000575 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000575 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PhanerochaeteV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:20508934
   |texte=   Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:20508934" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PhanerochaeteV1

This area was generated with Dilib version V0.6.37.
Data generation: Fri Nov 13 18:33:39 2020. Site generation: Fri Nov 13 18:35:20 2020

	Serveur d'exploration sur le phanerochaete
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le phanerochaete

Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.

Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki